pericyte medium with growth supplement and 2% fbs  (ScienCell)

Journal: Scientific Reports

Article Title: Multicellular Self-Assembled Spheroidal Model of the Blood Brain Barrier

doi: 10.1038/srep01500

Figure Lengend Snippet: (Trans-well): Is currently the standard setup where the BECs are grown on a filter insert with small pores to allow permeability studies between the two chambers. In co-culturing conditions, pericytes in normally grown on the opposite side of the filter potentially allow some cell-cell interaction through the pores and the astrocytes are grown in the bottom of the lower chamber to possibly influence the other cells by secreted factors into the media. (Matrigel): Cells are seeded in a gel structure to support migration an assembling. In this setup the BECs form tube-like structures on which pericytes preferentially adhere to, while the astrocytes are more loosely attached to the cell junctions. (Spheroidal): This model is completely independent of any supportive scaffold material for cell organization. The cells are allowed to freely self-assemble based on the intrinsic properties of each cell type. In our novel MCS-BBB setup, three key cell types of the neurovascular unit spontaneously form the spheroidal structure. The BECs form an outer cell monolayer representing the lumen side. The astrocytes accumulate in the core of the spheroids that could be perceived as the parenchyma space. Finally, pericytes line up in between to mediate a three cellular layer arrangement reproducing the morphological arrangement of the different cell types in the BBB.

Article Snippet: HpPs were grown in pericyte growth supplemented medium (ScienCell, USA) and hpAs were grown in astrocyte growth supplemented medium (ScienCell, USA).

Techniques: Permeability, Migration